Iman Kassem Saad

Associate professor
Chemistry - Biochemistry department - Section I - Hadath
Speciality: Chemistry
Specific Speciality: كيمياء حياتية

1986 - 1989: PhD

Claude Bernard University Lyon 1
Conductimetric dosage of ethanol and ethanal

1985 - 1986: DEA

Claude Bernard University Lyon 1
Study of the chymotrypsin

1984 - 1985: DESS

Claude Bernard University Lyon 1
Study of acetylcholinesterase

Publications 3 publications
Zouein FA, Duhé RJ, Arany I, Shirey K, Hosler JP, Liu H, Saad I, Kurdi M, Booz GW. Loss of STAT3 in mouse embryonic fibroblasts reveals its Janus-like actions on mitochondrial function and cell viability. Cytokine 2014

STAT3 has been implicated in mitochondrial function; however, the physiological relevance of this action is not established. Here we studied the importance of STAT3 to the cellular response to stimuli, TNFα and serum deprivation, which increase mitochondrial reactive oxygen species (ROS) formation. Experiments were performed using wild type (WT) and STAT3 knockout (KO) mouse embryonic fibroblasts (MEF). Both WT and STAT3 KO MEF expressed similar levels of tumor necrosis factor receptor 1 (TNFR1) and exhibited comparable IκBα degradation with TNFα. However, in the absence of STAT3 nuclear accumulation of NFκB p65 with TNFα was attenuated and induction of the survival protein c-FLIPL was eliminated. Nonetheless, WT MEF were more sensitive to TNFα-induced death which was attributed to necrosis. Deletion of STAT3 decreased ROS formation induced by TNFα and serum deprivation. STAT3 deletion was associated with lower levels of complex I and rates of respiration. Relative to WT cells, mitochondria of STAT3 KO cells released significantly more cytochrome c in response to oxidative stress and had greater caspase 3 cleavage due to serum deprivation. Our findings are consistent with STAT3 being important for mitochondrial function and cell viability by ensuring mitochondrial integrity and the expression of pro-survival genes.

Duffy P., Saad I, Wallach JM. New development of conductimetry in analytical biochemistry. Fresenius Z. fur Analytische Chemie 1988

Duffy P., Saad I, Wallach JM. New development of conductimetric measurements in analytical biochemistry. Analytica Chimica Acta 1988