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Molecular Diagnostics: molecular biology and functional genomics techniques and methods applied in molecular diagnostics Description

Key Elements

Code

DMAH 524

Formation

M2 Human Molecular Diagnostics

Semester

1

Credits

5

Number of Teaching Hours

32

Number of Tutoring Sessions

12

Number of Laboratory Sessions

0

Content

Objective

Content

Introduction: past, present and future of molecular diagnostics Molecular biology techniques used in molecular diagnostics DNA and RNA extraction from samples. Different amplification types: PCR (simple and multipex)/ NASBA/OLA/ bDNA/HCA (hybrid capture assay)/ Invader technolgy. Polymorphism analysis by RFLP. Footprinting using for pathogen detection. Specific mutation detection by ARMS, ASO and reverse ASO, COP. Genetic variation detection by chemical or enzymatic cleavage. Mutation detection by SSCP and HA Capillary electrophoresis analysis (CE). Mutation detection by DGGE and TGGE. Real-time PCR in molecular diagnostics. Minimal residual disease (MRD) detection by HRM. Functional genomic techniques used in molecular diagnostics Pyrosequensing. MLPA. DNA methylation analysis. Microarrays. Genomic deletion and duplication detection NGS Molecular and genomic cytogenetics ISH, FISH interphase, I-FISH, FIBER-FISH, PRINS, scFICH, CGH and CGH arrays, Tutorial classes consist of paper analysis for every technique or method.