Introduction: past, present and future of molecular diagnostics
Molecular biology techniques used in molecular diagnostics
DNA and RNA extraction from samples.
Different amplification types: PCR (simple and multipex)/ NASBA/OLA/ bDNA/HCA (hybrid capture assay)/ Invader technolgy.
Polymorphism analysis by RFLP.
Footprinting using for pathogen detection.
Specific mutation detection by ARMS, ASO and reverse ASO, COP.
Genetic variation detection by chemical or enzymatic cleavage.
Mutation detection by SSCP and HA
Capillary electrophoresis analysis (CE).
Mutation detection by DGGE and TGGE.
Real-time PCR in molecular diagnostics.
Minimal residual disease (MRD) detection by HRM.
Functional genomic techniques used in molecular diagnostics
DNA methylation analysis.
Genomic deletion and duplication detection
Molecular and genomic cytogenetics
ISH, FISH interphase, I-FISH, FIBER-FISH, PRINS, scFICH, CGH and CGH arrays,
Tutorial classes consist of paper analysis for every technique or method.