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General Biotechnology Description

Key Elements

Code

BIOV 424

Formation

M1 Environmental and life Sciences - Option : Vegetal

Semester

2

Credits

4

Number of Teaching Hours

36

Number of Tutoring Sessions

0

Number of Laboratory Sessions

0

Content

Objective

The main objectives of this course are to enable students: - To understand the interaction between science-technology - economy within a society. Discussions on the impact of technology in the evolution of societies. - Acquire all the basic techniques for the optimization of recombinant pharmaceutical protein: production of therapeutic molecules or drugs, vaccines, probes pros molecular diagnostics... by different biological systems used in biotechnology of industrial sector (bioreactors E. coli and yeast, stem cells and animal cells in culture, biopharming (transgenic animals and plants). techniques used in evolutionary metabolism. Intervention by the techniques of molecular biology on the metabolome, the fluxonome. - To have an introduction to the ethical and social aspects of biotechnology.

Content

Ch 1: Introduction: Biological, social and economic evolution. / Interaction science-technology, government market/ Introduction of GG in pharmaceutical / biotech market: experiences from Eli Lilly, Genentech, Kabi (Sweden) Ch 2: Optimization of production p r c/o E coli: The specialized vectors to optimize the synthesis of recombinant proteins [p. r.] (The eight factors) for the purification of p. r. for the solubilization of p. r.; for export p. r. , Polyvalent vectors. Promoters - cassettes - fused genes. / Problems of production of p r Ch 3: expression systems in eukaryotic cells: Overexpression in fungi: the benefits of system S. cerevisiae / expression in animal cells in culture: mammalian cells, ES cells, stem cells, cell ... insect cells. / Pharming: c / o animals. Ch 4: expression systems in plants. Application the technology to plants: Theme 1: Transgenesis technology in plants. Plant improving: plant expressing its own insecticide expression of δ endotoxin c / o but in the chloroplast; expression of glyphosate (herbicide) Theme 3: Transgenic strategies for the inhibition of genes: RNAi technologies: the example of tomato (anti ripening) / inactivation polygalactofuronase / inactivate ethylene synthesis. Theme 4: Problems of GMO: Biosafety and environment. Ch 5: Application of technology in medicine: Theme 1: the nucleic acid sequences as a diagnostic tool Theme 2: Production of p. r. referred to pharmaceuticals: new drugs: insulin / growth hormone human / factor VIII. Theme 3: recombinant vaccines (different types). Theme 4: modification of metabolism. Aa change at p. r. level to change the biochemical properties of the p. r. (active site, substrate recognition, Km, Kd, ...). Intervention in the metabolome fluxonome level. Basal and evolutionary metabolism Ch 7: technology applied to functional genomics The TD Analyses of articles The LAB Transformation of E. coli with the gene encoding GFP / cultures of recombinant clones for plasmid minipreps / agarose gel electrophoresis / protein affinity chromatography GFP / SDS PAGE / Western blot. Evaluation ad Ponderation. During BIOV 424: (1) Course partial exam: 30% and (2) final course exam: 70% BIOV LAB 425: (1) Project to be submitted by powerpoint by 2-3 students: 50% and (2) work tutorial sessions 50%. References: - Principes de Génie Génétique: (2004) Primrose/ Twyman/ Old second edition, éditons de Boeck - Molecular Biotechnology: (2003) Glick/ Pasternak third edition, ASM press Washington, DC.